Futurity.org ? Tool sprays brain to find tumors quickly

PURDUE (US) ? A new tool that sprays a stream of charged solvent onto tissue may one day help brain surgeons find and remove tumors.

?We hope to eventually be able to perform this analysis during surgery to help guide brain surgeons so that the borders of tumors can be identified and the cancer status of a site can be established before any tissue is removed,? says R. Graham Cooks, professor of chemistry at Purdue University.

?We aren?t there yet, but this was a critical step in the process. It shows we?ve found easily identifiable molecular patterns that can be used to diagnose the type and concentration of cancer cells,? Cook adds.

Purdue researchers designed the tool and collaborated with researchers and physicians at Brigham and Women?s Hospital at Harvard Medical School to perform the study. A paper detailing the results is published online in the Proceedings of the National Academy of Sciences.

?This approach could lead to real-time, image-guided surgery without interference with surgical care and without the administration of labeling agents,? says Nathalie Agar of Brigham and Women?s Hospital, who co-led the study. ?Such extensive and detailed information about the tissue was previously unavailable to surgeons and could lead to more precise tumor removal. In addition, having access to a detailed diagnosis on the day of surgery could help the oncologist more efficiently design the course of adjuvant therapy.?

Brain tumors are particularly challenging to remove, says co-author Alexandra Golby of Brigham and Women?s Hospital?s neurosurgery department.

?Tumor tissue within the brain often closely resembles normal brain tissue and may have indistinct boundaries, so it is difficult to determine where the tumor ends and the normal brain tissue begins,? says Golby. ?We want to preserve as much functional brain tissue as possible, especially when a tumor is in a critical area of the brain, like that which supports movement, speaking, or vision.?

Current surgical methods rely on the surgeon?s trained eye with the help of an operating microscope and imaging from scans performed before surgery.

Pathological examination of specimens taken from the brain during surgery provides the most specific information about the tissue and diagnosis of the cancer. However, this examination of frozen sections takes about half an hour, which is too long for it to be useful in examining multiple samples and guiding surgery, says Sandro Santagata, a pathologist at Brigham and Women?s Hospital who participated in the research.

Assessment in seconds

?The new tool is able?in a matter of seconds?to identify and classify glioma and meningioma tumors and recognize the tumor grade,? Santagata says. ?It is able to establish the same type of assessment the pathology offers, but at a pace that is compatible with surgery.?

Surgeons removed 32 specimens from the patients during surgery, which were later analyzed by both the new tool and standard pathology methods to test its accuracy. The results for the patient samples were in very good agreement with standard pathology, Cooks says.

The tool relies on an ambient mass spectrometry analysis technique developed by Cooks and his colleagues called desorption electrospray ionization, or DESI.

Mass spectrometry works by first turning molecules into ions, or electrically charged versions of themselves, so that they can be identified by their mass. Through mass analysis of the ions the contents of a sample can be identified.

Conventional mass spectrometry requires chemical separations, manipulations of samples, and containment in a vacuum chamber for ionization and analysis. The DESI technique eliminates these requirements by performing the ionization step directly on surfaces outside of the mass spectrometers, making the process much simpler, faster, and more applicable to surgical settings.

The researchers used DESI to evaluate the distribution and amounts of fatty substances called lipids within the brain tissue. A software program the team developed then used the results to characterize the brain tumors and detect boundaries between healthy and cancerous tissue. The researchers identified lipid patterns that corresponded to the different types and grades of cancer and concentrations of tumor cells through earlier studies of banked brain tumor tissue.

Livia Eberlin, who was a graduate student at Purdue at the time of the study and participated in the research, says the team expanded and improved the classification system for brain cancers by adding the meningioma tumor type, prior to the study.

More detailed analysis

?The classifier includes the two most common types of brain tumors, gliomas and meningiomas, which combined account for about 65 percent of all brain tumors,? says Eberlin, who is now a postdoctoral researcher at Stanford University. ?The molecular information that is obtained from this kind of imaging technology allows for an analysis that is much more detailed than what other techniques can offer. We hope that it can one day help the thousands of people affected by brain cancer every year.?

The classification results for brain tumor type in the validation studies of banked tissue agreed with standard pathology methods 100 percent of the time. The researchers found that the results of the mass spectrometry analysis of samples taken from the patients during surgery agreed with pathology with very few exceptions, despite the complexity and heterogeneity of the surgical samples. The composition of samples taken from different regions of an individual tumor can differ and tumor cell concentrations are especially variable.

The team plans to continue to add to and improve the classification software and to develop a miniature mass spectrometer that could be used during surgery, Cooks says. The team also will continue to examine the molecular patterns of cancerous tissue.

?Ambient ionization mass spectrometry allows us to look directly at unmanipulated tissue, just as a surgeon does, and get simple but extremely valuable molecular information,? Cooks says. ?These molecules have a story to tell not just in terms of aiding diagnosis, but also perhaps in terms of prognosis and our understanding of this devastating disease.?

The National Institutes of Health, James S. McDonnell Foundation, Brain Science Foundation and the Daniel E. Ponton Fund for the Neurosciences, and the Klarman Family Foundation funded the research.

Source: Purdue University

Source: http://www.futurity.org/health-medicine/tool-sprays-brain-to-find-tumors-quickly/

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